LOCUS       ISCR1-blaPER-1_unit     7838 bp    DNA     linear       22-OCT-2020
DEFINITION  Providencia rettgeri.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Providencia rettgeri
  ORGANISM  Providencia rettgeri
            Unclassified.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-blaPER-1_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Providencia rettgeri"
                     /mol_type="genomic DNA"
     mobile_element  1..7838
                     /gene="ISCR1-blaPER-1 unit"
                     /product="Putative resistance unit: ISCR1-blaPER-1 unit"
     mobile_element  complement(1..2154)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   2134..2154
                     /gene="oriIS"
                     /product="ISCR1 oriIS"
     mobile_element  complement(2155..2182)
                     /gene="ISCR1 remnant"
                     /product="Insertion sequence: ISCR1 remnant"
     repeat_region   2162..2182
                     /gene="oriIS"
                     /product="ISCR1 oriIS"
     CDS             2245..3171
                     /gene="blaPER-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase PER-1"
                     /translation="MNVIIKAVVTASTLLMVSFSSFETSAQSPLLKEQIESIVIGKKA
                     TVGVAVWGPDDLEPLLINPFEKFPMQSVFKLHLAMLVLHQVDQGKLDLNQTVIVNRAK
                     VLQNTWAPIMKAYQGDEFSVPVQQLLQYSVSHSDNVACDLLFELVGGPAALHDYIQSM
                     GIKETAVVANEAQMHADDQVQYQNWTSMKGAAEILKKFEQKTQLSETSQALLWKWMVE
                     TTTGPERLKGLLPAGTVVAHKTGTSGIKAGKTAATNDLGIILLPDGRPLLVAVFVKDS
                     AESSRTNEAIIAQVAQTAYQFELKKLSALSPN"
     CDS             3245..3820
                     /codon_start=1
                     /transl_table=11
                     /product="Maleylacetoacetate isomerase / Glutathione
                     S-transferase"
                     /translation="MQLLGSVASPFVRRLRLVLAGQPYQFVALNIFESEGRSVLVQHN
                     PARKVPVLVDGEQVIFDSGVIYRYLASKLKFKPLSWDQENGLTTINACTDSLVELLLC
                     KRSGFDVTEDKLFFNLQHERIQATLEALEQQIRAGHFGDWDYPGISLFTLIDWILFRD
                     LVDLKPFPVLLQFRNAHLNRPMVAETDPRLS"
     CDS             complement(3859..5700)
                     /gene="mdlB"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC MDR transporter/ATP-binding cassette (ABC)
                     transporters"
                     /translation="MLGFFERLTNPFPDKEPTQPPQGLFAFCLHYSQGMVLPLILMSI
                     ATALLAALEVSLFGYMGQLVDWLVTKNPATFWQEEQDTLITMAIVMLLVLPGLVFFHS
                     ALIHQTILGNYPMSIRWLAHRYLLKQSLGFYQNEFAGRIATKVMQTALAVRETATKLL
                     DVMVYVVVYFGSMLYLIADADWRLVLPMIAWLVIYTGLQFYFVPRLKQVATEQADARS
                     EMTGRIVDSYTNISTIKLFSHTARESEYARDSMQLFLVPVYRQMRLATWLNICVQGLN
                     YLLVFSIATLAIWLWSVNAVTVGSIAIAVSLALRLNGMSQWIMWEVSALFENIGTVAD
                     GIATLSQPQQIIDKADAKPLEVPQGQIEFKQLNFNYGKAAGEHGPVIDGLDLSIKPGE
                     KVGLVGRSGAGKSTLVNLLLRFYDVNSGQILIDGQDIKTVSQESLRAHIAMVTQDTSL
                     LHRTVRENILYGRPGATEAEMIEAARLAEADAFIQELTDPKGNSGYDAQVGERGVKLS
                     GGQRQRIAIARVLLKNAPILILDEATSALDSEVESAIQHSLTQLMIGKTVIAIAHRLS
                     TIAAMDRLIVLDQGRIVEQGSHQELIAKGGIYAQLWAHQTGGFIGVE"
     CDS             6071..6772
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MDAKELLVYMKQAELQAELVVEAGQQLDRAIKQLHDWSEDWDCQ
                     RRACEDMYDAVHLILIHSYQLSRIFWPAKCCGELGLDLCHRAMALRSEINLPDLNHPL
                     RNEALWRHADELDHSVRDNTALRRYQAHHLTGFNQVITWMDNEAMFCWLEPASKTFVF
                     HAEEFALAQLLDAVHQVQQDIQNFLNKQHQSNLKVAKAGVHHYEKQWGAFKIRQDRSC
                     PQLCCYDYLSNLINP"
     CDS             complement(6745..7074)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MLTFVMSAITFGFLLLSLFFYKKLIGMSDALNIIEKQVAADMEI
                     RAHRLCLLAYEAQRFGNSVDRRALDEEFKDFLHLYIEDYQAEVAKKIREHKLSEISAY
                     GFIKLDK"
     CDS             complement(7573..7803)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MQINLNTPGTAATSTIQTVAPAAQAKTTTTTAPSTADTVTLSAE
                     AKAKLAAEQQESKAINPTLTTLGGGWGNEPPK"
BASE COUNT     2156 a   1865 c   1816 g   2001 t
ORIGIN
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat acccaaaaag
     2161 gtggtttata cttcctatac cctaaaaagg cagctttggc tgccttttgt ataattcata
     2221 tcgacttatc aaaaggacaa tccgatgaat gtcattataa aagctgtagt tactgcctcg
     2281 acgctactga tggtatcttt tagttcattc gaaacctcag cgcaatcccc actgttaaaa
     2341 gagcaaattg aatccatagt cattggaaaa aaagccactg taggcgttgc agtgtggggg
     2401 cctgacgatc tggaaccttt actgattaat ccttttgaaa aattcccaat gcaaagtgta
     2461 tttaaattgc atttagctat gttggtactg catcaggttg atcagggaaa gttggattta
     2521 aatcagaccg ttatcgtaaa cagggctaag gttttacaga atacctgggc tccgataatg
     2581 aaagcgtatc agggagacga gtttagtgtt ccagtgcagc aactgctgca atactcggtc
     2641 tcgcacagcg ataacgtggc ctgtgatttg ttatttgaac tggttggtgg accagctgct
     2701 ttgcatgact atatccagtc tatgggtata aaggagaccg ctgtggtcgc aaatgaagcg
     2761 cagatgcacg ccgatgatca ggtgcagtat caaaactgga cctcgatgaa aggtgctgca
     2821 gagatcctga aaaagtttga gcaaaaaaca cagctgtctg aaacctcgca ggctttgtta
     2881 tggaagtgga tggtcgaaac caccacagga ccagagcggt taaaaggttt gttaccagct
     2941 ggtactgtgg tcgcacataa aactggtact tcgggtatca aagccggaaa aactgcggcc
     3001 actaatgatt taggtatcat tctgttgcct gatggacggc ccttgctggt tgctgttttt
     3061 gtgaaagact cagccgagtc aagccgaacc aatgaagcta tcattgcgca ggttgctcag
     3121 actgcgtatc aatttgaatt gaaaaagctt tctgccctaa gcccaaatta acagactatc
     3181 agcactatct aagccgctga ctctggttgt acactaaagc tatggtttga aattggagta
     3241 ggttatgcag ttattaggtt cagtggcttc cccttttgtt cgtcgtttac gtttagtact
     3301 ggcagggcaa ccttatcagt ttgtagcgct taatattttt gagtctgaag gccgttcagt
     3361 gttggtacaa cataatccgg cacgcaaagt gcctgtgtta gtggatggag agcaggttat
     3421 ttttgattca ggcgtaattt atcgttattt ggcttcgaaa ctgaaattca aaccattgag
     3481 ctgggatcag gaaaacggtc tgacgaccat caatgcctgc acagactcct tggttgaatt
     3541 actgctttgt aagcgctcag gttttgacgt gactgaagat aaattgtttt ttaatcttca
     3601 gcatgagcga attcaggcga cgctagaggc acttgagcaa caaatcaggg cagggcattt
     3661 cggtgactgg gactatcctg gtatcagtct gttcacttta atcgactgga tattgttccg
     3721 cgatttagtc gacttaaaac ccttccctgt attattacag ttcagaaatg cgcatttaaa
     3781 ccggccgatg gtcgctgaaa ccgacccgcg tttaagctaa aaacaacagg tcagagccaa
     3841 gactctgacc tttattattt attccacacc aataaagccg ccggtttgat gcgcccataa
     3901 ttgagcgtaa ataccgcctt tggcgatcag ttcctgatga ctgccttgtt ccacaatacg
     3961 accttgatcc agaacgatca gcctgtccat agctgctatg gtagataaac ggtgtgcaat
     4021 ggctataacc gttttgccta tcatcaactg agtcagacta tgctgaatag ctgattctac
     4081 ttcggagtcc agcgctgagg tggcttcatc caaaatcaga ataggggcgt ttttcagtaa
     4141 aacccgagct attgcaatac gttgacgttg accaccagaa agcttgactc cacgttcgcc
     4201 cacctgagcg tcgtagccac tgttgccttt aggatcggta agctcctgaa taaaagcatc
     4261 ggcttcggct aaacgggctg cttcaatcat ttccgcttcg gtagcgccag gacgaccata
     4321 taaaatattt tcccttacag tacggtgtag cagggaggta tcctgggtca ccatagctat
     4381 atgagcgcga agactttcct gtgatacagt tttgatgtcc tgaccgtcta tcaaaatctg
     4441 gcctgaattg acatcataaa agcgcagcag taagttcacc agcgtggatt taccagcacc
     4501 agaacggccg actaaaccta ctttttcacc gggtttaatc gacaagtcca gaccatcaat
     4561 cacaggacca tgttcacctg ctgctttgcc gtagttaaag ttcagttgct taaactcaat
     4621 ctgaccttgt ggcacttcaa gcggcttcgc atcagctttg tcgatgattt gctgaggctg
     4681 agacaaagtc gcaataccat ctgccacagt acctatgttt tcaaacagtg cactgacttc
     4741 ccacatgatc cactgcgaca taccatttaa acgcagcgcc agactgactg cgatggcaat
     4801 agagcccaca gtgacggcat tgacagacca aagccatatc gccagcgtgg caatagagaa
     4861 caccaacaga tagttcagac cctgcacaca aatattgagc caggtagcca gtcgcatttg
     4921 tctgtacaca ggcaccagaa acagctgcat gctatcgcgg gcgtattctg actcacgagc
     4981 cgtgtgtgaa aacagtttaa tagtgctgat attggtgtag ctgtcgacaa tacggcctgt
     5041 catttctgaa cgggcatcgg cttgttctgt tgcaacttgt ttcagtctcg gcacaaaata
     5101 aaactgcaag ccggtgtata tgactaacca ggcaatcatc ggcagtacca agcgccaatc
     5161 tgcatcggcg atcagataca acatagagcc aaaatacacc accacataca ccatcacatc
     5221 cagcagcttg gtggccgttt cgcgcactgc caaagccgtt tgcatcactt tagtggcgat
     5281 gcgaccagcg aattcattct ggtagaaacc caaactttgt ttcagtaaat aacgatgcgc
     5341 cagccagcgg atcgacatag ggtagttacc caatatagtt tggtggatca gggctgagtg
     5401 aaaaaatacc aaaccaggca acaccagcaa catcactatg gccatagtga tcaaggtatc
     5461 ctgctcttcc tgccagaaag tagccggatt cttggttacc aaccaatcga ctaattgccc
     5521 catatagcca aataaggaca cttctaatgc ggccagtaag gctgtcgcaa tagacatcag
     5581 gatcagaggc aacaccatgc cttgactgta atgcaaacaa aatgcaaaca ggccttgagg
     5641 tggctgagtt ggctctttat cagggaaagg gttggttaag cgttcaaaaa agcccagcat
     5701 aaaattcctc tgcagcaacg aaaaaggcag ttattgtacg tcaaagttta aaaaacagat
     5761 gtaactattt gatgaaagcc cggtttagaa cggccatctt attttataac cgatagcttg
     5821 tgactcgctt ttttgctgaa cagaacagca gattgttgaa aataaataca aaaaatctga
     5881 aaaatccaaa agatgctata tcacatgaac tcagcagctt agatttattg aaggaccgtt
     5941 aataggtaat aactggaaaa tctataactt agcttctgtt ttgtcagctt gcaaaggact
     6001 ttttgctttc tacactgaaa atacagagta cccgaatcag ggtgttgtct cgtcttacag
     6061 gagtgttgaa atggatgcca aagaattatt ggtctacatg aagcaggcag aactgcaagc
     6121 tgagttagtc gttgaggctg gtcagcaact ggacagagcc atcaaacaat tacatgactg
     6181 gtctgaagac tgggattgtc agcgccgggc ttgtgaagat atgtacgatg ccgtgcattt
     6241 aattctgatc catagctatc aattatcccg gattttctgg cccgccaagt gttgcggaga
     6301 actggggctg gatttgtgtc atagagccat ggcgctgcga agtgaaatca acctgcctga
     6361 tctaaaccat ccactgcgga atgaggcgtt atggcgtcat gcagatgagc tggatcattc
     6421 agtgcgggat aatactgctc tgcgtcgtta tcaagcgcat catttaaccg gttttaatca
     6481 ggtgatcacc tggatggata atgaagccat gttttgctgg cttgaacctg ccagtaaaac
     6541 ctttgtgttt catgccgagg agtttgcttt agcccaatta ctggatgctg tgcatcaggt
     6601 tcagcaagat attcagaatt ttcttaacaa gcagcatcag tcaaatctaa aggtggccaa
     6661 agctggcgtg catcactatg agaagcagtg gggggcattt aaaataagac aagacagatc
     6721 ttgtccacag ctttgttgtt atgactactt atctaattta ataaatccgt aggcagaaat
     6781 ctcgctgagc ttatgttctc tgatcttttt tgcgacttct gcttggtagt cttcaatgta
     6841 caggtgcaaa aaatctttaa actcttcatc caaagcacgt ctatccacag agttaccgaa
     6901 ccgttgcgct tcgtatgcaa gcaggcataa tctatgcgcc cggatctcca tatcagcggc
     6961 cacctgcttt tcgataatgt ttaatgcatc tgacattcca atcaacttct tgtagaaaaa
     7021 cagacttaac agtaaaaagc caaacgtaat tgcagacata acaaaagtca gcattcctga
     7081 agttcctttc tgtgcttgac ggctcctgat atggaccata aaaatatcgc taaaattgct
     7141 gcgatattta tagctggtat tccatatttg tatataccac ccaatagatc agtgccaaat
     7201 atgactctgt ctgtgaatct cgctacctgc agaacagagt gcatcaagat gcttaaacaa
     7261 atgaattgag tgatttgttc aaccgcccat acgaattttc tatgaataac ccagatgata
     7321 ccaagcgtca atgtttgaca tatcacccaa cttgggtacc aaagtaagcg agcgagctca
     7381 ggagatgttt cagatatccc catcagcaaa ggagcaatcc tgtcttgagc taatcccgat
     7441 aaagtgagta tcaatatggt tattcgtgca gatttactgg ccgaccagac taaggacagc
     7501 agtagagaac ctatgcacaa cacccataag tactcagcaa gagtcgtaat aaatgtcccc
     7561 acaaactacc tcttattttg gtggttcatt accccagcca ccacctaatg tagtcagtgt
     7621 tggatttatt gctttagatt cttgctgctc tgcagccagc ttcgcctttg cttcagcaga
     7681 taaggtgaca gtgtctgcag tagagggtgc tgttgtagtg gtggttttag cctgagcagc
     7741 aggagcaaca gtttgtattg ttgatgtagc cgctgtgccc ggagtattta agttgatttg
     7801 cataactgtt ccttaccatt gtcactaaat cagaaaac
//
